Garlic Increases Antioxidant Levels in Diabetic and Hypertensive Rats Determined by a Modified Peroxidase Method
نویسندگان
چکیده
Oxidative damage by free radicals has been implicated in the pathogenesis of vascular disease in diabetes and hypertension. In the present study, the total antioxidant status in diabetic and hypertensive rats before and after treatment with garlic (Allium sativum) was determined. The total serum antioxidants were measured by a modified method reported earlier by Miller and coworkers. The reproducibility of the assay was confirmed by determining standard curves for the known antioxidants: trolox (a stable analog of vitamin E), glutathione and vitamin C with interassay correlation coefficients (R(2), n = 10 in triplicate) of 0.9984, 0.9768 and 0.987, respectively, confirming the reliability and reproducibility of the assay. This assay was then used to determine total serum antioxidant levels of streptozotocin-induced diabetic rats and two-kidney one-clip hypertensive rats both before and after 3 weeks of treatment with an aqueous extract of garlic (500 mg/kg IP daily). The serum antioxidant levels of rats after 3 weeks of treatment were significantly higher (P < .001) than the pretreatment levels in both diabetic and hypertensive rats. The increased serum antioxidant levels were paralleled by a decrease in serum glucose in the garlic-treated diabetic rats and lowered systolic blood pressure in the garlic-treated hypertensive rats. We conclude from our study that (i) total antioxidants can be measured by a simple, reproducible, reliable assay and (ii) the total antioxidant status can be significantly improved by treatment with garlic.
منابع مشابه
Biochemical and Histological Study of Protective Effect of Sodium Tungstate on Oxidative Stress Induced by Streptozotocin in Pancreas of Diabetic Rats
Purpose: The aim of this study was evaluation of effects of sodium tungstate on oxidative stress in pancreas of streptozotocin induced diabetic rats. Materials and Methods: Sixty mature Wistar male rats (Age: 2-3 month) were selected and divided into six groups randomly (n=10). Control (C), STZ-induced diabetic (D), STZ-induced diabetic rats were treated by sodium tungstate (1 mg/ml) in drinkin...
متن کاملEffects of Three Medicinal Plants Extracts in Experimental Diabetes: Antioxidant Enzymes Activities and Plasma Lipids Profiles in Comparison with Metformin
In the present study we aimed to evaluate the effects of methanolic extracts of the bulbs of Garlic (Allium sativum L., Alliaceae) and Persian shallot (Allium ascalonicum L., Alliaceae ) and leaves of Sage (Salvia officinalis L., Lamiaceae ), ASE, AAE and SOE respectively, on the antioxidant enzymes such as superoxide dismutase (SOD), glutathione peroxidase (GPX) and catalase (CAT) activities an...
متن کاملEffects of Three Medicinal Plants Extracts in Experimental Diabetes: Antioxidant Enzymes Activities and Plasma Lipids Profiles in Comparison with Metformin
In the present study we aimed to evaluate the effects of methanolic extracts of the bulbs of Garlic (Allium sativum L., Alliaceae) and Persian shallot (Allium ascalonicum L., Alliaceae ) and leaves of Sage (Salvia officinalis L., Lamiaceae ), ASE, AAE and SOE respectively, on the antioxidant enzymes such as superoxide dismutase (SOD), glutathione peroxidase (GPX) and catalase (CAT) activities an...
متن کاملبهبود وضعیت آنتیاکسیدان و استرس اکسیداتیو در موشهای صحرایی دیابتی تحت درمان با ژل رویال
Background and Objective: Diabetes mellitus is one of the most common metabolic disorders worldwide. Numerous natural compounds have been developed to treat diabetes mellitus. Royal jelly (RJ) has antioxidant and antidiabetic properties. This study was carried out to investigate the effects of RJ on antioxidant status, lipid peroxidation and liver tissue in diabetic rats. Materials and Metho...
متن کاملتغییر فعالیت گلوتاتیون پراکسیداز گلبول قرمز و میزان آنتیاکسیدان تام پلاسما در اثر عصاره Securigera Securidaca در موشهای صحرایی دیابتی
Background & Aim: Reactive oxygen species can affect many cellular functions through protein oxidation or initiation of lipid peroxidation cascade. The study was designed to investigate changes in erythrocyte glutathione peroxidase activity and plasma total antioxidant level due to Securigera Securidaca extract in diabetic rats. Material and Methods: The present experimental study was car...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
دوره 2011 شماره
صفحات -
تاریخ انتشار 2011